2026 Fluorescent Fish (GloFish): Comprehensive Care, Species, and Breeding Guidelines

Fluorescent fish, commercially recognized as GloFish, represent one of the most striking advancements in biotechnology applied to the aquarium hobby. Contrary to popular belief, these specimens are not artificially dyed; they are genetically modified organisms (GMOs) that express fluorescent proteins (GFP, RFP) derived from jellyfish and coral. This distinctive glow is a hereditary trait, fully visible under actinic light, and demands specific filtration conditions alongside a carotenoid-enriched diet to preserve its vitality.

The popularity of “glowing fish” in home aquaria has grown exponentially due to their vibrant aesthetic. By integrating cnidarian genes, species such as zebrafish and betta fish have transformed the concept of aquatic decor, offering a chromatic spectacle under specialized lighting. Currently, high demand in the ornamental market has prompted research centers and corporations to diversify species offerings through transgenic approaches (Barman et al., 2019). However, the origin of these organisms was purely scientific; initially, transgenic fish were developed for human therapeutic production, environmental monitoring, and as experimental models in biology and aquaculture (Van Eenennaam & Olin, 2006).

The first specimens were created in Singapore with the objective of detecting environmental toxins. Researchers at the National University of Singapore successfully inserted jellyfish genes into zebrafish, subsequently discovering the immense potential of this technology for the pet industry. Today, breeding fluorescent fish not only satisfies enthusiasts but also enhances the decorative value of professional aquariums (Aleksandrov, 2020), revitalizing the aquatic ornamental sector with innovative options. This article analyzes the various types of fluorescent fish, their care requirements, the accessories that enhance their brilliance, and their comprehensive impact on science and commerce.

Key Takeaways: Essential Facts About Fluorescent Fish

• Genetic, Not Artificial, Origin: Fluorescent fish (such as GloFish) are neither dyed nor injected; their color results from the transgenesis of jellyfish and coral proteins, making their glow both natural and hereditary.
• Lighting is the Secret: Unlike bioluminescence (self-generated light), these fish require an actinic or blue light source to “activate” their glow. Under standard white light, their colors appear dull.
• Species Variety: Currently, biotechnology has expanded to include species such as zebrafish, black tetra, tiger barb, rainbow shark, betta, medaka, and angelfish.
• Behavior and Health: Genetic modification does not alter their social behavior, aggression levels, or feeding habits; however, certain species (like the Tiger Barb) may be more sensitive to abrupt environmental changes.
• Strategic Nutrition: A diet rich in carotenoids and bloodworms is fundamental not only for their health but also for maximizing the intensity of their red and orange pigments.
• Responsibility and Biosafety: While biological reproduction is possible, it is legally prohibited due to patents. Furthermore, they must never be released into the wild, as they could destabilize local ecosystems.
• Innovative Future: Utilizing techniques such as CRISPR, the industry expects the development of sterile varieties (to protect the environment) and unprecedented colors within the ornamental market.

What are Fluorescent Fish? Science and Biological Differentiation

Fluorescent fish, commonly referred to as “glowing fish,” are transgenic organisms in which genes from marine species—such as jellyfish and anemones—have been integrated to enable the expression of fluorescent proteins. Under the incidence of ultraviolet (UV) or actinic light, these specimens manifest a vibrant luminescence that highlights their morphology within the aquarium.

It is essential to distinguish between bioluminescence and fluorescence, terms that are often confused. While bioluminescence is a natural chemical process through which an organism generates its own light (common in deep-sea species), fluorescence in these ornamental fish is the result of the genetic insertion of exogenous proteins that react to an ambient light source.

Types of Fluorescence and Their Genetic Origin

The application of transgenesis in the aquarium hobby has allowed for the diversification of species’ chromatic palettes (Cebeci et al., 2020). Researchers primarily employ four protein variants to obtain different phenotypes:

• Green Fluorescence: Originated by the GFP (Green Fluorescent Protein) transgene, extracted from the deep-sea jellyfish Aequorea victoria.
• Red Fluorescence: Produces pinkish and reddish hues through the RFP (Red Fluorescent Protein) transgene, derived from the coral Discosoma sp. and the anemone Entacmaea quadricolor.
• Yellow Fluorescence: Responsible for orange tones, based on the YFP (Yellow Fluorescent Protein) transgene.
• Cyan Fluorescence: Achieved through the CFP (Cyan Fluorescent Protein) transgene.

Each of these proteins features specific structures that often include optimized modifications relative to their wild-type progenitors (Thanh et al., 2014).

Biofluorescence in the Natural Environment

Beyond modified organisms, biofluorescence is an intrinsic phenomenon in nature. Sparks et al. (2014) initially documented over 180 biofluorescent species in coral reefs. Recent research by Carr et al. (2025) has expanded this catalog, identifying 48 new species and bringing the total to 459, distributed across 87 taxonomic families.

Furthermore, Schramm and Weiß (2024) classify natural bioluminescence into three mechanisms: bacterial symbiosis, the coelenterazine system (of cnidarian origin), and the use of vargulin (derived from ostracod crustaceans).

How Were Fluorescent Fish or GloFish Created?

Although research into modified organisms began in the 1980s, the first significant milestone occurred in 1995 with the development of the transgenic zebrafish (Danio rerio) capable of expressing green fluorescent protein (Gong et al., 2001). This breakthrough laid the groundwork for what we now know as the glowing fish industry.

Originally, GloFish were not conceived as ornamental pets but as biotechnological tools to monitor pollution in river ecosystems. However, their aesthetic potential did not go unnoticed. In 2003, after obtaining an official license from the National University of Singapore, Yorktown Technologies began marketing the red phosphorescent zebrafish in the United States under the registered trademark GloFish. Since then, these specimens have maintained sustained popularity among North American aquarists (Schramm & Weiß, 2024).

Technically, the first specimens were obtained through the strategic insertion of fluorescent protein transgenes directly into the animal’s muscle tissue (Scotto & Serna, 2013). This technique ensured that the glow was an integral characteristic of the organism rather than a superficial treatment. Today, the transgenesis of fluorescent proteins is a well-established practice in the ornamental fish trade, used to diversify color morphs in various tropical species (Leggatt & Devlin, 2020). The GloFish catalog has evolved significantly: in addition to the original zebrafish, the current selection includes varieties of black tetras, tiger barbs, and rainbow sharks, all available in a wide range of phosphorescent colors.

Fluorescent vs. Conventional Fish: Key Differences and Considerations

While fluorescent fish share much of their biology with traditional species, their maintenance and aesthetics present distinct nuances that every aquarist should know. Below, we break down the primary differences:

• Infrastructure Requirements: Although their biological needs (water parameters and diet) are similar to those of their non-modified counterparts, maximizing their visual potential requires additional investment. To fully appreciate their fluorescence, specialized lighting and UV-reactive decorations are indispensable—elements that are not strictly necessary for conventional fish.

• Visual Impact and Aesthetics: The main distinction lies in their luminescence. While conventional fish rely on light reflecting off their scales, fluorescent fish possess an intrinsic glow that intensifies under specific LED lighting systems (actinic or blue light). This effect creates a vibrant, futuristic atmosphere that traditional fish cannot replicate in similar decorative settings.

• Dynamics and Compatibility: In terms of behavior, many fluorescent varieties—such as tetras or barbs—tend to be remarkably active. This vitality can create a stressful environment for more sedentary or timid conventional species. It is essential to plan the aquarium community by prioritizing species with compatible energy levels to ensure ecosystem harmony.

Species Diversity: Types of Fluorescent Fish and Their Attributes

The GloFish trademark has significantly expanded its catalog, incorporating species with diverse biological profiles and behaviors; however, other companies have also developed various transgenic fish varieties. For proper aquarium management and accurate semantic mapping, it is essential to distinguish between commercial varieties and their corresponding scientific names:

Fluorescent Zebrafish (Danio rerio)

The fluorescent zebrafish holds the distinction of being the first transgenic organism developed for strictly scientific purposes, specifically within the fields of human health and ecotoxicology. Nevertheless, its undeniable visual appeal allowed researchers to quickly identify its disruptive potential within the aquarium industry. Today, these specimens have consolidated their presence in the global market through a sophisticated array of chromatic variations.

Under the GloFish brand, the zebrafish is marketed in a palette that includes blue, electric green, purple, pink, red, and orange. Innovation in this field remains ongoing; recent research, such as that by Ossa-Hernández et al. (2023), highlights the potential of the Red Fluorescent Protein (encoded by the DsRed gene and derived from the coral Discosoma sp.) to intensify and refine the phenotypic coloration of Danio rerio, further elevating the species’ aesthetic standards.

Transgenic Black Tetras or Monjita Fish (Gymnocorymbus ternetzi)

The development of fluorescent varieties in the Black Tetra (Gymnocorymbus ternetzi) represents a milestone in the expansion of modern fishkeeping. Although the first specimens with red fluorescence were documented by Pan et al. (2008), technology has evolved to produce organisms that integrate two specific transgenes. These allow the fish to exhibit high-intensity phosphorescent green coloration under various lighting conditions, including white, ultraviolet, and blue light (DFO, 2018).

One of the most relevant points for the management of this species is its biological and social viability. In this regard, Leggatt and Devlin (2020) analyzed the competitive foraging capacity and thermal tolerance of the transgenic green tetra. The results are revealing: genetic modification does not alter foraging success nor does it influence the aggressive behavior of individuals in either juvenile or adult stages.

Thanks to these advancements, the GloFish brand currently offers a wide chromatic range of tetras, spanning shades of blue, electric green, purple, pink, red, and orange, consolidating them as a vibrant and biologically stable option for the community aquarium.

Fluorescent Tiger Barbs (Puntius tetrazona)

Within the GloFish brand catalog, the Tiger Barb has established itself as one of the most striking species, marketed in a vibrant palette that includes blue, electric green, purple, pink, red, and orange. However, its maintenance requires more specialized attention compared to other varieties.

Cutting-edge research conducted by Lindstrom and Volkoff (2025) suggests that these transgenic strains may exhibit increased susceptibility to external factors. According to the authors, these specimens tend to be more sensitive to food restriction and fluctuations in their environmental conditions. These findings underscore the importance of maintaining stable water parameters and a consistent diet to ensure the well-being and longevity of these fish in the home aquarium.

Fluorescent Rainbow Shark (Epalzeorhynchos frenatum)

The Rainbow Shark, also commonly known as the Green Labeo (Epalzeorhynchos frenatum), is a freshwater cyprinid native to the river ecosystems of Southeast Asia. In its wild state, this species is noted for a robust morphology that can reach up to 16 cm in length, characterized by a body in grayish tones with bluish nuances that contrast vividly with its deep red fins.

Building upon this aesthetic foundation, the GloFish brand has developed varieties that enhance its visual appeal. Currently, the Rainbow Shark is marketed in a wide range of fluorescent colors, including blue, electric green, purple, pink, red, and orange. These transgenic versions maintain the imposing silhouette of the original species, becoming the centerpiece of any large-scale aquarium.

Glowing betta fish (Betta splendens)

Glowing Bettas represent an immensely popular option, merging their emblematic territorial nature with the innovative appeal of fluorescence. This species, renowned for its vast chromatic diversity, finds in genetic modification a visual enhancement that highlights its distinguished presence within the aquatic ecosystem. Due to their nature, they are ideal specimens for smaller-scale aquaria, provided that decorations and hiding spots are supplied to ensure their environmental comfort and security.

Currently, under the GloFish brand, both male and female specimens are marketed in a vibrant electric green hue. By integrating this variety, the aquarist gains the sophistication of the Betta splendens behavior coupled with the luminosity of transgenic technology, creating an unparalleled focal point under the appropriate lighting.

Fluorescent Marine Medaka (Oryzias dancena)

The fluorescent marine Medaka (Oryzias dancena) established itself within the sector thanks to the successful integration of the cyan fluorescent protein gene (Thanh et al., 2014). One of its most valuable traits for the aquarist is its euryhaline nature, which allows it to adapt and thrive with equal efficiency in both freshwater aquaria and marine environments.

Innovation in this species continues to set scientific milestones. Recently, Huang et al. (2025) managed to develop a stable line of transgenic marine Medaka (Oryzias melastigma) with a fundamental characteristic: sterility. These specimens express the mCherry red fluorescent protein, regulated by the zebrafish mylz2 promoter. The result is a high-intensity purple/red coloration, fully visible to the naked eye, combining extraordinary visual impact with responsible biosafety management.

Fluorescent Angel Fish (Pterophyllum scalare)

The Angelfish, or Scalare, is one of the most sophisticated additions to the fluorescent organism market. Although its commercialization is already a reality, the exact biotechnological techniques employed for its creation remain under industrial trade secrecy. In this field, the company Jy Lin Trading set a precedent by announcing the development of the first angelfish with electric green luminescence.

Parallel to commercial activity, the scientific community has documented its own achievements. Thanh et al. (2022) reported the successful transgenesis of Pterophyllum scalare specimens through the integration of red fluorescent protein. This advancement not only expands the chromatic palette available to hobbyists but also reaffirms the viability of genetic manipulation in high-value ornamental cichlids.

Bright Koi and Carp Fish

Bright koi and carp fish offer an alternative for those seeking large fluorescent fish. Although they are more difficult to find and care for in home aquariums, their presence is impressive.

Can Fluorescent Fish Breed at Home?

One of the most frequent questions among aquarists is whether fluorescent fish can reproduce in captivity. The technical answer is affirmative, although its execution involves a legal complexity that should not be underestimated. Biologically, species such as fluorescent Danios and Tetras retain their instincts and follow spawning rituals identical to those of their wild counterparts.

The Intellectual Property Conflict

Despite their reproductive capacity, in many countries, breeding for commercial purposes or distribution is strictly prohibited by patent laws. When purchasing a GloFish specimen, the consumer enters into an implicit agreement for exclusively ornamental and personal use. Since the “genetic design” is the intellectual property of biotechnology corporations, marketing offspring can lead to significant legal sanctions for patent infringement.

Research Findings and Behavior

While there is a theory that these organisms are designed to be sterile, scientific evidence suggests otherwise:

• Reproductive Viability: Documented experiments by Scotto and Chuan (2018) confirmed that the reproduction of the first genetic lines of zebrafish (TK1) is entirely possible.
• Environmental Impact: A concerning finding was reported by Barroso et al. (2022), who identified the reproduction of transgenic specimens in natural environments within the Brazilian Amazon basin, posing challenges for biosafety.
• Sexual Selection: In terms of competitiveness, Howard et al. (2015) observed that wild-type males are often aggressively superior to transgenic males during courtship, which could influence population dynamics in shared environments.

Tank Setup: Maximizing the Fluorescent Fish Spectrum

Designing the ideal environment for transgenic fish transcends conventional fishkeeping. To ensure both animal welfare and maximum visual impact, it is imperative to configure an ecosystem that enhances their biotechnological properties.

Aquarium Dimensions and Capacity

Tank volume must be determined based on the selected species. While a 40-liter (approx. 10-gallon) aquarium is sufficient for schools of small species like zebrafish or tetras, larger specimens—such as Angelfish or glowing Koi varieties—require significantly more spacious environments to ensure adequate swimming room and water parameter stability.

Aquascaping and Reactive Decoration

To create a cohesive aesthetic, it is recommended to integrate decorations specifically designed for fluorescence. Using dark substrates, rocks, and plants with reactive pigments intensifies the contrast, ensuring that both the environment and the specimens look spectacular under the correct lighting.

The Secret of Luminescence: Specialized Lighting

Many hobbyists experience a loss of brilliance upon bringing their fish home; this is generally due to an inadequate lighting configuration. It is vital to understand that fluorescence is not bioluminescence: the fish does not generate its own light in total darkness, but rather requires an external photon source to “excite” the fluorescent proteins housed within its tissue.

• Actinic Spectrum (Blue Light): This is the key to activating neon pigments. A cycle of 10 to 12 hours is recommended.

• Lighting Management: During the day, the use of white light favors the growth of live plants (essential for chemical balance), while “Moonlight” mode (actinic blue) highlights fluorescence during viewing hours.

• Technical Note: Avoid high-intensity white light typical of professional planted aquariums, as it tends to “wash out” the visual effect of fluorescent colors.

Pro-Tip: Although blacklight (UV) creates an extreme neon effect, its use should be limited. Prolonged exposure can stress the fishes’ photosensitive system and accelerate undesirable algae blooms.

Care Protocol: Health and Vitality of Fluorescent Fish

While fluorescent fish are noted for their resilience, their well-being and color intensity depend directly on rigorous maintenance and strategic nutrition. Environmental stability is the determining factor in preventing stress, which often dulls their luminescence.

Nutritional Strategy and Coloration

A balanced diet is the foundation of their development. Although they accept commercial flakes and pellets, the use of live or freeze-dried foods makes a significant difference in their quality of life. According to research by Rostika et al. (2024), nutritional objectives should be segmented based on the desired outcome:

• Growth Optimization: To maximize size and body weight (especially in varieties like Starfire Red), providing Daphnia sp. and Tubifex sp. is most effective.
• Chromatic Intensification: If the goal is to enhance the characteristic red color, bloodworms (Chironomidae) are established as the best option due to their supply of natural pigments.
• Maintenance Note: It is imperative to avoid overfeeding; excess decomposing organic matter rapidly deteriorates water chemistry.

Water Quality Management

Water transparency and purity are crucial for actinic light to penetrate correctly and highlight fluorescence. Ammonia, nitrite, and nitrate levels must be monitored regularly under the following parameters:

• Filtration System: An efficient filter capable of processing at least four times the total aquarium volume per hour is required. This ensures the removal of toxic nitrogenous compounds and keeps the water crystal clear.

• Temperature: Must be kept stable between 24°C and 27°C using an automatic heater with a thermostat.

• Potential of Hydrogen (pH): The optimal range fluctuates between 6.5 and 7.5, allowing for healthy compatibility with most schooling species.

The Global Transgenic Fish Market: Distribution and Leading Brands

Currently, the trade of tropical ornamental fish with phosphorescent properties has established itself as a global phenomenon. Despite regulatory restrictions and bans in force across various jurisdictions, these specimens maintain a significant presence in international markets.

The GloFish brand (owned by Spectrum Brands, Inc., USA) leads the sector as the most recognized authority. Its catalog encompasses high-demand species such as zebrafish, tetras, bettas, and rainbow sharks, distributed in a proprietary chromatic palette featuring trademarked names like “Starfire Red,” “Moonrise Pink,” “Sunburst Orange,” “Electric Green,” “Cosmic Blue,” and “Galactic Purple.” In the United States alone, these specimens are available in over 7,000 specialty stores, evidencing the massive success of this technology within the pet industry (Anderson, 2017).

Simultaneously, in the Asian market, Taikong Corp (Taiwan) plays a fundamental role, overseeing the marketing and distribution of the fluorescent Medaka. This diversification of suppliers underscores the expansion of biotechnology applied to the aquarium hobby, transforming the aesthetics of home aquaria on a worldwide scale.

Compatibility and Social Behavior

Aquarium coexistence depends closely on the ethology of each species. Most fluorescent fish, notably GloFish (Tetras and Danios), possess a gregarious nature and a sociable temperament; therefore, their well-being increases significantly when maintained in schools of six or more individuals.

In contrast, the glowing Betta retains the strongly territorial character of its original lineage. For these varieties, it is imperative to design a habitat that incorporates abundant hiding spots and strategic decorations to act as visual barriers. This type of environmental enrichment not only reduces stress-induced cortisol levels but also minimizes territorial conflicts, ensuring either harmonic coexistence or optimal development in individual tanks.

Applications and Versatility: From Aesthetics to Biomonitoring

The impact of fluorescent fish transcends recreational fishkeeping, establishing them as valuable tools across various scientific disciplines and environmental design.

Ornamentation and Aquascaping

In domestic and exhibition settings, these specimens have revolutionized the concept of “living decor.” Their ability to provide vibrant colors and unprecedented shades allows hobbyists to customize high-impact artificial ecosystems. The synergy between the fish and light-reactive accessories enables the creation of dynamic environments that enhance fluorescence, transforming the aquarium into a visual spectacle of technological precision.

Applications in Scientific and Biomedical Research

Beyond their aesthetic appeal, these organisms originally emerged as genetic markers designed to evaluate ecosystem health. The zebrafish (Danio rerio) remains the preferred model for understanding the interaction of chemical substances with biological systems.

This line of research remains at the forefront of genetics and biomedicine. A recent example is the development led by Xie et al. (2023), who created a specific transgenic zebrafish line for the detection and monitoring of dioxins and dioxin-like compounds (DLCs) in the environment. Such advancements demonstrate that fluorescence is, in reality, a critical biological sensor for the protection of biodiversity and public health.

Applications and Uses of Fluorescent Fish

Fluorescent fish are not only used in decorative aquariums but also have scientific and research applications.

Aquarium Decoration

Fluorescent fish are popular in home and exhibition aquariums, where they add color and novelty. The growing supply of fluorescent decorations for aquariums allows for personalized environments that enhance bioluminescence and create a fascinating visual spectacle.

Scientific Research

Fluorescent fish were originally developed as genetic markers to track ecosystem health. Fluorescent zebrafish, for example, help scientists better understand the effects of certain substances on the environment. This line of research is still thriving and contributes to advancements in biomedicine and genetics. For instance, Xie et al., (2023) developed a transgenic zebrafish line to monitor dioxins/dioxin-like compounds (DLC) in the environment.

Risks, Ethics, and Biosafety: The Debate Over Transgenic Fish

The proliferation of genetically modified ornamental fish has sparked intense global debate. The primary concern lies in their potential release into natural ecosystems and the unpredictable impact this could have on indigenous populations and wild food chains. Due to this potential risk, several countries maintain strict regulations prohibiting their sale and cultivation.

Philosophical and Ethical Considerations

From a critical perspective, Hung (2026) posits that biotechnology is transforming animals into “designed commodities” or objects of aesthetic consumption. This approach deeply questions current regulatory frameworks and the ethical implications of commodifying life through gene editing.

Vulnerability and Survival Capacity

Science has rigorously examined whether these fish could survive and compete in the wild:

• Predation: Hill et al. (2011) determined that fluorescent zebrafish are twice as vulnerable to predators (such as largemouth bass) as their wild-type counterparts, as their glow makes them easy targets.
• Thermal Resistance: Research by Dugan et al. (2024) demonstrates that these fish are unlikely to establish self-sustaining populations in cold climates. In regions such as Canada, specimens lose their equilibrium at 13.14°C and die before reaching the 4°C typical of winter.

Innovations in Biosafety and Environmental Evidence

To mitigate risks, Chu et al. (2023) highlight that editing the dead end gene (dnd1) allows for the induction of reproductive loss of function (sterility), a key alternative to prevent accidental propagation.

Despite agencies like the FDA and studies by McGowan and Leggatt (2021) concluding that environmental risk is minimal or equivalent to that of conventional fish, reality demands caution. Moutinho (2022) reported cases in Brazil where specimens escaped from aquaculture farms have managed to multiply in Atlantic forest streams, underscoring the necessity for responsible ownership.

Human Safety

Regarding public health, Dugan et al. (2024) evaluated four commercial GloFish lines (GPM2021, RPM2022, OPM2021, and PPM2021), concluding that there is no evidence of risks or adverse effects on human health derived from their use in home aquaria.

Responsibility Note: Under no circumstances should fluorescent fish be released into natural bodies of water. The stability of our ecosystems depends on the ethics and commitment of every aquarist.

Conclusion

The emergence and consolidation of fluorescent fish represent an unprecedented milestone at the intersection of biotechnology and the aquarium hobby. What began as a scientific tool for environmental biomonitoring has evolved into a global commercial phenomenon, democratizing access to genetically modified organisms within the home setting. This transition has not only diversified the aesthetics of modern aquaria but has also driven cutting-edge research in genetics and animal behavior, demonstrating that fluorescence is a property as fascinating as it is useful for contemporary science.

However, the success of species such as GloFish carries an inescapable responsibility for both the industry and the aquarist. Although current scientific evidence—supported by agencies like the FDA and recent studies from 2024 and 2025—suggests that environmental and human health risks are minimal under controlled conditions, cases of escapes into vulnerable ecosystems underscore the need for strict biosafety. The implementation of genetic sterilization technologies is emerging as the definitive solution to harmonize the enjoyment of these “designed commodities” with the preservation of indigenous biodiversity.

Ultimately, owning a fluorescent fish is an act that must transcend mere visual appreciation. The owner’s commitment is fundamental: providing a technologically adequate habitat that enhances their vitality and, above all, ensuring that these specimens never reach natural water sources. By integrating scientific knowledge with ethical and responsible ownership, the fishkeeping community can continue to enjoy this chromatic spectacle without compromising the ecological balance of our planet.

Frequently Asked Questions About Fluorescent Fish

References

Aleksandrov Y. New solution–fluorescent fish aquariums located in building elements and furnitures of the skyscraper Kun Min, China. Bulgarian Journal of Agricultural Science, 2020 – agrojournal.org

Anderson W. 2017. Austin company behind glow-in-the-dark fish in pet stores sells IP for $50 million. Austin Business Journal.

Barman, H. K., Rasal, K. D., & Mondal, S. (2019). Status and prospects of gene editing and transgenic in fishes. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 79(Sup-01), 292–299.

Barroso Magalhães André Lincoln, Marcelo Fulgêncio Guedes Brito & Luiz Gustavo Martins Silva (2022) The fluorescent introduction has begun in the southern hemisphere: presence and life-history strategies of the transgenic zebrafish Danio rerio (Cypriniformes: Danionidae) in Brazil, Studies on Neotropical Fauna and Environment, DOI: 10.1080/01650521.2021.2024054

Carr, E.M., Martin, R.P., Thurman, M.A. et al. Repeated and widespread evolution of biofluorescence in marine fishes. Nat Commun 16, 4826 (2025). https://doi.org/10.1038/s41467-025-59843-7

Cebeci Ayse, Ilhan Aydin, Anthony Goddard. 2020. Bigger, stronger, better: Fish transgenesis applications and methods. Biotech Studies 29(2), 85-97 http://doi.org/10.38042/biost.2020.29.02.05

Chu, W., Huang, S., Chang, C., Wu, J., & Gong, H. (2023). Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of the dnd1 gene by CRISPR/Cas9 genome editing. Frontiers in Genetics, 14, 1029200. https://doi.org/10.3389/fgene.2023.1029200

Dashrath Rasal Kiran, Vemulawada Chakrapani, Swagat Kumar Patra, Arun S Ninawe, Jitendra Kumar Sundaray, Pallipuram Jayasankar1 and Hirak Kumar Barman. 2016. Status of Transgenic Fish Production with Emphasis on Development of Food Fishes and Novel Color Varieties of Ornamental Fish: Implication and Future Perspectives. Journal of FisheriesSciences.com

DFO. 2018. Environmental and Indirect Human Health Risk Assessment of the Glofish® Electric Green® Tetra and the Glofish® Long-Fin Electric Green® Tetra (Gymnocorymbus ternetzi): A Transgenic Ornamental Fish. DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2018/027.

Dugan, S., Ali, K., and Demissie, Z. 2024. Indirect Human Health Risk Assessment of GloFish® Electric Green® (GPM2021), GloFish® Starfire Red® (RPM2022), GloFish® Sunburst Orange® (OPM2021), and GloFish® Galactic Purple® (PPM2021) Pristella Tetras (Pristella maxillaris): Transgenic Ornamental Fishes. DFO Can. Sci. Advis. Sec. Res. Doc. 2024/044. iv + 20 p.

Gong, Z., Ju, B. & Wan, H. Green fluorescent protein (GFP) transgenic fish and their applications. Genetica 111, 213–225 (2001). https://doi.org/10.1023/A:1013796810782

Hill Jeffrey E., Anne R. Kapuscinski & Tyler Pavlowich (2011) Fluorescent Transgenic Zebra Danio More Vulnerable to Predators than Wild-Type Fish, Transactions of the American Fisheries Society, 140:4, 1001-1005, DOI: 10.1080/00028487.2011.603980

Howard, R.D., Rohrer, K., Liu, Y. and Muir, W.M. (2015), Mate competition and evolutionary outcomes in genetically modified zebrafish (Danio rerio). Evolution, 69: 1143-1157. https://doi.org/10.1111/evo.12662

Huang, J., Cai, Y., Huang, Z., & Chen, S. X. (2025). Generation of sterile transgenic fluorescent marine medaka by genetic manipulation. Aquaculture Reports, 45, 103114. https://doi.org/10.1016/j.aqrep.2025.103114

Hung, R.Y.Y. (2026). Glowing Entanglements: GloFish, Biotechnology, and the Ethico-Politics of Engineered Life. In: Animal Lives through Five Centuries of Art and Science. Palgrave Studies in Animals and Literature. Palgrave Macmillan, Cham. https://doi.org/10.1007/978-3-032-05977-2_7

Leggatt, R.A., Devlin, R.H. Fluorescent protein transgenesis has varied effects on behaviour and cold tolerance in a tropical fish (Gymnocorymbus ternetzi): implications for risk assessment. Fish Physiol Biochem 46, 395–403 (2020). https://doi.org/10.1007/s10695-019-00725-3

Lindstrom, A., & Volkoff, H. (2025). Endocrine regulation of feeding in non-transgenic and transgenic fluorescent orange tiger barb (Puntigrus tetrazona). General and Comparative Endocrinology, 367, 114730. https://doi.org/10.1016/j.ygcen.2025.114730

McGowan, C., and Leggatt, R. 2021. Environmental Risk Assessment of the GloFish® Galactic Purple® and Cosmic Blue® Danios: Transgenic Ornamental Fish. DFO Can. Sci. Advis. Sec. Res. Doc. 2021/005. viii + 32 p.

Moutinho Sofia. 2022. Transgenic fish invades Brazilian streams. Science.

Noble Brzezinski, S., Leggatt, R., Johnson, N., and McGowan, C. 2021. Environmental Risk Assessment of the Glofish® Sunburst Orange® Danio: a Transgenic Ornamental Fish imported to Canada for sale in the pet trade. DFO Can. Sci. Advis. Sec. Res. Doc. 2021/013. viii + 35 p.

Ossa-Hernández, N., Marins, L.F., Almeida, R.V. et al. Red Fluorescent Protein Variant with a Dual-Peak Emission of Fluorescence. Mar Biotechnol 25, 1099–1109 (2023). https://doi.org/10.1007/s10126-023-10262-z

Pan, X.; Zhan, H.; Gong, Z. 2008. Ornamental Expression of Red Fluorescent Protein in Transgenic Founders of White Skirt Tetra (Gymnocorymbus ternetzi). Marine Biotechnology 10: 497–501.

Rostika, R., Priowirjanto, G. H., Zidni, I., & Dewi, A. M. (2024). Growth and Color Brightness of Glofish Fed Natural and Artificial Diets In Cianjur District, West Java Province. DEVOTION Journal of Research and Community Service, 5(8)

Schramm, S., & Weiß, D. (2024). Bioluminescence – The Vibrant Glow of Nature and its Chemical Mechanisms. ChemBioChem, 25, e202400106. https://doi.org/10.1002/cbic.202400106

Scotto, Carlos; Serna, Fernando. 2013. Primera identificación molecular del transgen de la proteína fluorescente roja (RFP) en peces Cebra (Danio rerio) transgénicos ornamentales introducidos en el Perú. Scientia Agropecuaria 4: 257-264.

Scotto Espinoza, Carlos, & Chuan García, Ricardo. (2018). Cruzamiento y flujo génico de los transgenes de las proteínas fluorescentes roja (RFP) y verde (GFP) en el pez cebra transgénico (Danio rerio) introducido al Perú. Scientia Agropecuaria, 9(3), 417-421. https://dx.doi.org/10.17268/sci.agropecu.2018.03.13

Sparks JS, Schelly RC, Smith WL, Davis MP, Tchernov D, Pieribone VA, et al. (2014) The Covert World of Fish Biofluorescence: A Phylogenetically Widespread and Phenotypically Variable Phenomenon. PLoS ONE 9(1): e83259. https://doi.org/10.1371/journal.pone.0083259

Thanh Vu Nguyen, Young Sun Cho, Sang Yoon Lee, Dong Soo Kim and Yoon Kwon Nam. 2014. A Cyan Fluorescent Protein Gene (cfp)-Transgenic Marine Medaka Oryzias dancena with Potential Ornamental Applications. Fish Aquat Sci 17(4), 479-486, 2014

Thanh Vu Nguyen, Bui Hoang Loc, Nguyen Hoang Thuy Vy et al. Red Fluorescent Protein Expression in Transgenic Founder of Angelfish (Pterophyllum sp) Driven by Zebrafish Myosin Light Chain 2 Promoter, 12 January 2022, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-1187734/v1

Van Eenennaam Alison L. and Paul G. Olin. 2006. Careful risk assessment needed to evaluate transgenic fish. CALIFORNIA AGRICULTURE • VOLUME 60, NUMBER 3

Xie, S., Yang, B., Li, S., Ge, L., Li, M., Chen, Q., Qing, X., & Zou, J. (2023). Generation and application of a novel transgenic zebrafish line Tg(GAcyp1a:EGFP/Luc) as an in vivo assay to sensitive and specific monitoring of DLCs in the environment. Ecotoxicology and Environmental Safety, 264, 115471. https://doi.org/10.1016/j.ecoenv.2023.115471

Milthon Lujan

Editor at the digital magazine AquaHoy. He holds a degree in Aquaculture Biology from the National University of Santa (UNS) and a Master’s degree in Science and Innovation Management from the Polytechnic University of Valencia, with postgraduate diplomas in Business Innovation and Innovation Management. He possesses extensive experience in the aquaculture and fisheries sector, having led the Fisheries Innovation Unit of the National Program for Innovation in Fisheries and Aquaculture (PNIPA). He has served as a senior consultant in technology watch, an innovation project formulator and advisor, and a lecturer at UNS. He is a member of the Peruvian College of Biologists and was recognized by the World Aquaculture Society (WAS) in 2016 for his contribution to aquaculture.